rabbit anti human integrin v polyclonal antibody  (Proteintech)

Journal: Molecular Cancer

Article Title: In vivo optical imaging of integrin α V -β 3 in mice using multivalent or monovalent cRGD targeting vectors

doi: 10.1186/1476-4598-6-41

Figure Lengend Snippet: (A) Western blot analysis of expressions of integrin subunits α V , β 1 and β 3 in HEK293(β 1 ) and HEK293(β 3 ) cell lines. (B) Confocal laser scanning microscopic images of HEK293(β 1 ) and HEK293(β 3 ) cells incubated for 30 min at 37°C in the presence of 0.1 μM Cy5-labeled RAFT-c(-RGDfK-) 4 , cRGD, or RAFT-c(-RβADfK) 4 . Nuclei were stained with Hoechst 33342 (blue), and fluorescence signal from Cy5 was pseudocolored red. Original objective: Plan-Neofluar 40x/1.30 Oil ph3.

Article Snippet: The membranes were then incubated with primary antibody: rabbit anti-human integrin α V polyclonal antibody (1:5000; Chemicon International, Inc., Temecula, CA), rabbit anti-integrin β 1 tail serum (1:1500; kindly provided by Dr C. Albiges-Rizo, Grenoble, France) or mouse anti-human β 3 monoclonal antibody (clone VI-PL2, 1:100; BD Biosciences PharMingen, San Diego, CA).

Techniques: Western Blot, Incubation, Labeling, Staining, Fluorescence